Key features and details | |
Cat. No. | MABL-1478 |
Name | Anti-Eimeria acervulina mAbs |
Clone No. | AFD- 5D11 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IFA, immunoblot, ELISA, IF |
Species Reactivity | E. acervulina, E. maxima |
Basic Information | |
Specificity | This antibody recognises an antigen on the apical tip of E. acervulina and E. maxima sporozoites. |
Alternative Name | 5D-11 |
UniProt | |
Immunogen | This antibody was raised by immunising chickens with CD8+ T cells pre-adsorbed with E. acervulina soluble sporozoite antigen. |
Application Notes | This antibody has been used in confocal laser immunofluorescence analysis to stain Eimeria acervulina and E. maxima sporozoites, but will not stain merezoites (Constantinoiu et al, 2003). An scFv derived from the variable regions of this antibody has been used in ELISA, IFA and immunoblot assays (Kim et al, 2001) |
Antibody First Published | Kim et al. Generation and Characterization of Recombinant ScFv Antibodies Detecting Eimeria acervulina Surface Antigens Hybridoma. 2001 Jun;20(3):175-81. PMID:11461666 |
Note on publication | Describes the derivation of an scFv from this monoclonal antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
