Key features and details | |
Cat. No. | MABL-1476 |
Name | Anti-EGFRvIII mAbs |
Clone No. | AFD-806 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, therapeutic, FC, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the human delta2–7 epidermal growth factor receptor (de2–7 EGFR or EGFRvIII) which is a truncated version of the EGFR commonly expressed in glioma. This antibody does not bind wild type EGF receptor. Studies have shown that it binds a cryptic epitope on the EGFR in the region between amino acids 287–302, and it is suggested that breaking the disulfide bond preceding the epitope might allow the CR1 domain to open up sufficiently for antibody binding. |
Alternative Name | de2–7 EGFR; EGF receptor; delta2–7 epidermal growth factor receptor; Epidermal Growth Factor Receptor variant III |
UniProt | P00533 |
Immunogen | The original antibody was generated by immunization of mice with NR6 mouse fibroblasts expressing the truncated de2–7 EGFR. |
Application Notes | This antibody binds the U87 MG glioma cell line transfected with the de2–7 EGFR but not the parental U87 MG cell line, which expresses the wt. EGFR without gene amplification in a flow cytometric analysis. This antibody significantly inhibited the growth of U87 MG xenografts transfected with the de2–7 EGFR in a dose-dependent manner using both preventative and established tumor models. It also inhibited U87 MG cells transfected with the wild-type EGFR, which increased expression to ;106 EGFRs/cell and mimics the situation of gene amplification. Xenografts treated with this antibody all displayed large areas of necrosis that were absent in control tumors. This antibody was also reported to y inhibit the growth of new and established A431 xenografts, a cell line expressing >106 EGFRs/cell. This antibody was also used for the immunoprecipitation of de2–7 EGFR from U87 MG.D2–7 cells. |
Antibody First Published | Luwor et al. Monoclonal antibody 806 inhibits the growth of tumor xenografts expressing either the de2-7 or amplified epidermal growth factor receptor (EGFR) but not wild- type EGFR. Cancer Res. 2001 Jul 15;61(14):5355-61. PMID:11454674 |
Note on publication | Describes the generation of this antibody and examines the antitumor activity of this antibody against human tumor xenografts grown in nude mice. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
