Key features and details | |
Cat. No. | MABL-1469 |
Name | Anti-EGFR mAbs |
Clone No. | AFD- 7A7 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, FC, IF, IHC |
Species Reactivity | Human, Mouse |
Basic Information | |
Specificity | Recognizes the extracellular domain of EGFR. |
Alternative Name | ERBB; ERBB1; HER1; PIG61; mENA; avian erythroblastic leukemia viral (v- erb-b) oncogene homolog; cell growth inhibiting protein 40; cell proliferation-inducing protein 61; proto- oncogene c-ErbB-1; receptor tyrosine-protein kinase erbB-1; epidermal growth f |
UniProt | P00533 |
Immunogen | Recombinant extracellular domain of murine EGFR (rECD-mEGFR). |
Application Notes | The antibody was generated in order to establish a mouse model for in vivo pre-clinical evaluation of therapies targeting EGFR. The antibody was able to recognize the murine and human EGFR expressed on 3LL or A431 tumor cell lines by flow cytometry. Moreover, the antibody could immunoprecipitate EGFR of 3LL cell lysate, it was used in immunohistochemistry on mouse skin tissue and mammary adenocarcinoma, and it was able to detect EGFR in Western blots (Garrido et al., 2004; PMID: 15312307). The antibody inhibited EGF-induced EGFR signaling in D122 tumor cells by reducing EGFR phosphorylation as well as the phosphorylation of molecules involved in downstream EGFR signaling; the antibody showed strong anti-metastatic activity on D122 tumors, for which the contribution of T cells is essential (Garrido et al., 2007). The crystal structure of the Fab fragment of the antibody was determined. |
Antibody First Published | Garrido G, Sanchez B, Rodriguez HM, Lorenzano P, Alonso D, Fernandez LE. 7A7 MAb: a new tool for the pre-clinical evaluation of EGFR-based therapies. Hybrid Hybridomics. 2004 Jun;23(3):168-75. PMID:15312307 |
Note on publication | Describes the generation and characterization of an anti-murine EGFR extracellular domain monoclonal antibody (7A7 MAb) (IgG1). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
