Anti-Cytoplasmic dynein mAbs

Cat. No.

MABL-1347

Name

Anti-Cytoplasmic dynein mAbs

Clone No.

AFD-74.1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

IP, WB, IF

Species Reactivity

Fish, Sheep: Drosophila, Mouse

Basic Information

Specificity

This antibody is specific for the   intermediate chain of dynein. Dynein acts as one of several non-catalytic   accessory components of the cytoplasmic dynein 1 complex that are thought to   be involved in linking dynein to cargos and to adapter proteins that regulate   dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular   retrograde motility of vesicles and organelles along microtubules. The   intermediate chains mediate the binding of dynein to dynactin via its 150 kDa   component (p150-glued) DCTN1. May play a role in mediating the interaction of   cytoplasmic dynein with membranous organelles and kinetochores.

Alternative Name

Dynein light chain 1, cytoplasmic; Dynein   light chain LC8-type 1

UniProt

Q29RQ3

Immunogen

This antibody was raised by immunizing mice   with bovine brain cytoplasmic dynein.

Application Notes

The murine version of this antibody bound to   protein A-Sepharose beads is able to immunoprecipitate dynein. A western blot   on sucrose density purified dynein using the mouse version of this antibody   has proven the specificity of this antibody (Dillman and Pfister, 1994;   pmid:7528220). A western blot on the intermediate chain of dynein using the   mouse version of this antibody determined the interaction between the dynein   intermediate chain (IC) and the dynactin p150 (Siglin et al, 2013; pmid: 23577064). The ability of this antibody to inhibit the CC1/NudE-Dynein   interaction was determined. To do this the mouse version of this antibody was   incubated with rat brain purified cytoplasmic dynein and then mixed with   bacterially expressed GST-CC1 or GST-NudE. The results were analyzed using   coomassie- stained gels (McKenney et al, 2011; pmid: 21911489). The influence   of this antibody on microtubule polymerization was determined in vitro. Cells   were grown on cover slips and then incubated with the murine version of this   antibody (Zhapparova et al, 2007; pmid: 18205606).

Antibody First Published

Dillman and Pfister Differential   phosphorylation in vivo of cytoplasmic dynein associated with anterogradely   moving organelles J Cell Biol. 1994 Dec;127(6 Pt 1):1671-81.PMID: 7528220

Note on publication

To determine if phosphorylation may play a   role in the regulation of cytoplasmic dynein, the in vivo phosphorylation   state of cytoplasmic dynein from two cellular pools was examined.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.