Key features and details | |
Cat. No. | MABL-1325 |
Name | Anti-cytochrome C mAbs |
Clone No. | AFD- R1D3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ICC |
Species Reactivity | Mouse |
Basic Information | |
Specificity | The antibody is specific for cytochrome c. The antibody binds to a variety of oxidatively modified cyt c species, such as nitrated at Tyr74 or oxidized at Met80, which lose the sixth heme ligation (Fe-Met80); it does not bind to several cyt c phospho- and acetyl-mimetics. |
Alternative Name | cyt c; Cytochrome c somatic |
UniProt | P62897 |
Immunogen | |
Application Notes | The antibody was employed to identify alternative conformations of cyt c in cells under oxidant- or senescence-induced challenge as confirmed by immunocytochemistry (Tomasina et al., 2022; PMID： 36378644). |
Antibody First Published | Tomasina et al. De novo sequencing and construction of a unique antibody for the recognition of alternative conformations of cytochrome c in cells Proc Natl Acad Sci U S A. 2022 Nov 22;119(47)：e2213432119. PMID：36378644 |
Note on publication | The paper describes the sequencing of the original antibody |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
