Key features and details | |
Cat. No. | MABL-1324 |
Name | Anti-Cytochrome C mAbs |
Clone No. | AFD- E8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA |
Species Reactivity | Horse (Equus caballus) |
Basic Information | |
Specificity | This antibody binds horse cytochrome c. This antibody recognizes three major discontiguous segments (33 to 39; 56 to 66; 96 to 104), and two minor sites on cyt c opposite to the exposed haem edge (PMID： 9698550). |
Alternative Name | CYC; CYCS; FabE8; Fab E8 |
UniProt | P00004 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with horse cytochrome c (cyt c) conjugated to hemocyanin. |
Application Notes | The original mouse IgG1 version of this antibody binds horse cytochrome c with an affinity of Kd ≃ 10(−9) M (PMID： 2993413). A solid phase radioimmunoassay was used to identify the specific epitope that the antibody binds (PMID： 2454225). The structural mechanism involved in the binding of the IgG1 antibody E8 to its highly charged protein antigen horse cytochrome c (cyt c) was studied using crystallographic structures (PMID： 9698550). |
Antibody First Published | Carbone et al. Monoclonal antibodies to horse cytochrome c expressing four distinct idiotypes distribute among two sites on the native protein. J Immunol. 1985 Oct;135(4)：2609-16. PMID：2993413 |
Note on publication | Describes the generated of 4 antibodies that bind horse cytochrome c. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
