Key features and details | |
Cat. No. | MABL-1210 |
Name | Anti-Circumsporoziote protein mAbs |
Clone No. | AFD- 2A10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, in vitro, in vivo, IP, ITC, WB, ELISA, IF |
Species Reactivity | Plasmodium falciparum |
Basic Information | |
Specificity | The antibody is specific for the repeat domain of CSP. Circumsporozoite protein (CSP) is a secreted protein of the sporozoite stage of the malaria parasite. |
Alternative Name | CSP; PfCSP; CS |
UniProt | P02893 |
Immunogen | The original antibody was generated by immunizing BALB/c mice with sporozoites of P.falciparum. |
Application Notes | The structure of the Fab fragment was determined by X-ray crystallography. Isothermal titration calorimetry (ITC) experiments were performed to understand the interaction between the antibody or the Fab fragment and CSP or the synthetic peptide antigen (NANP)6, which is a short segment of the antigenic NANP-repeat region of CSP. The binding free energies (ΔG) and dissociation constants (KD) were found to be -49.0 kJ/mol and 2.7 nM for the full antibody with CSP, -40 kJ/mol and 94 nM for the Fab with CSP, and -36.4 kJ/mol and 420 nM for the Fab with the (NANP)6 peptide, suggesting that the antigenic region of CSP constitutes a multivalent antigen. The specificity of the antibody to (NANP)6 peptide was confirmed by ELISA (Fisher et al., 2017; PMID: 28759640). The antibody inhibited in vitro inhibition of sporozoite invasion (Herrera et al., 2015; PMID: 26169272). |
Antibody First Published | Nardin et al. Circumsporozoite proteins of human malaria parasites Plasmodium falciparum and Plasmodium vivax J Exp Med. 1982 Jul 1;156(1):20-30. PMID:7045272 |
Note on publication | The paper describes the generation and characterization of a panel of monoclonal antibodies to circumsporozoite (CS) proteins. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
