Key features and details | |
Cat. No. | MABL-1208 |
Name | Anti-Chronic lymphocytic leukemia mAbs |
Clone No. | AFD- Lym-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vitro, in vivo, therapeutic, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody recognises a cell surface protein expressed in normal and malignant B lymphocytes. |
Alternative Name | CLL |
UniProt | |
Immunogen | The antibody was generated by immunizing BALB/c mice with chronic lymphocytic leukemia cell nuclei. |
Application Notes | Live cells radioimmunoassay techniques showed that the antibody bound to cell lines of B-cell lineage. B-cell specificity of the antibody was confirmed by immunofluorescence. Flow cytometric analysis of lymphoma biopsies showed the majority of B-cell tumors were detected by the antibody.Immunoperoxidase staining of frozen sections of human lymphoid tissues showed that the antibody stained germinal center and mantle zone B-lymphocytes as well as interfollicular histiocytes (Epstein et al. 1987; PMID: 3542194). Surface expression of the antigen recognized by the original version of the antibody was confirmed by flow cytometry in RL and DB, human diffuse large-cell lymphoma lines; TU-2C and CHIM-62, EBV-induced human B-cell lymphoma cell lines; Raji and Daudi, human Burkitt's lymphoma cell lines; and Michel and Karpas 299, ALCL cell lines. |
Antibody First Published | Epstein et al. Two new monoclonal antibodies, Lym-1 and Lym-2, reactive with human B-lymphocytes and derived tumors, with immunodiagnostic and immunotherapeutic potential Cancer Res. 1987 Feb 1;47(3):830-40. PMID:3542194 |
Note on publication | The paper describes the generation and characterization of the antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
