Key features and details | |
Cat. No. | MABL-1207 |
Name | Anti-Cholera enterotoxin B subunit mAbs |
Clone No. | AFD- TE33 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | RIA, Western blot, ELISA |
Species Reactivity | Vibrio cholerae |
Basic Information | |
Specificity | This antibody binds the CTP3 loop of the B subunit of the cholera toxin. This antibody is not capable of binding cholera toxin in its native form or its wild type form. TE33 specifically recognizes a loop comprising the sequence 'VPGSQHID' and a beta-turn formed by the sequence 'VPGS'. The CTP3 peptide is involved in saccharide binding to the receptor on the epithelial cells. |
Alternative Name | ctxB; toxB; VC_1456; Cholera enterotoxin subunit B; Cholera enterotoxin B chain; Cholera enterotoxin gamma chain; Choleragenoid |
UniProt | P01556 |
Immunogen | The original antibody was generated by immunizing mice with CTP3 which is a conserved loop comprising amino acid residues 50-64 on the surface of the B subunit. CTP3 is involved in saccharide binding to the receptor on epithelial cells. |
Application Notes | The original IgG1 antibody which was raised against the CTP3 peptide also cross reacts with cholera toxin in a solid phase ELISA. The binding of the original IgG1 antibody to cholera toxin and H- LT, a heat-labile toxin produced by enterotoxigenic strains of E. coli was done using solid-phase RIA and western blot (PMID: 2450576). In solution, the original antibody has a thousandfold lower affinity for the cholera toxin as compared to that for CTP3 (PMID: 1379072). The binding of the original antibody to both CTP3 peptide and cholera toxin was assayed by ELISA, solid-phase radioimmunoassay and immunoblot assay. The antibody was incapable of binding cholera toxin in its native form when tested in a solution- phase assay system (PMID: 1993848). |
Antibody First Published | Anglister et al. NMR study of the complexes between a synthetic peptide derived from the B subunit of cholera toxin and three monoclonal antibodies against it. Biochemistry. 1988 Jan 26;27(2):717-24. PMID:2450576 |
Note on publication | The contact interaction between a synthetic peptide CTP3 (residues 50-64 of the B subunit of cholera toxin) suggested as a possible epitope for synthetic vaccine against cholera and three different anti-peptide monoclonal antibodies were studied by nuclear magnetic resonance (NMR). |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
