Key features and details | |
Cat. No. | MABL-1136 |
Name | Anti-CD81 mAbs |
Clone No. | AFD- Ab10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallization, FACS, inhibition, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the large extracellular loop of human CD81. |
Alternative Name | CD81 antigen; 26 kDa cell surface protein TAPA-1; Target of the antiproliferative antibody 1; Tetraspanin-28 (Tspan-28) |
UniProt | P60033 |
Immunogen | The original antibody was generated by immunization of Balb/c and Naval Medical Research Institute (NMRI) mice with CD81 extracellular loop 2 (ECL2) protein. |
Application Notes | The scFv version of this antibody is based on an anti-CD81 clone whose binding specificity was determined via FACS that was successfully used in an inhibition assay against HCV (Ji et al., 2015; PMID： 25417967). The exact binding site of this antibody was determined via crystallization (Nelson et al., 2018; PMID： 29778602). The scFv version of this antibody was used successfully in FC (Susa et al., 2020; PMID： 32338599). |
Antibody First Published | Nelson et al. Structure-Guided Combinatorial Engineering Facilitates Affinity and Specificity Optimization of Anti-CD81 Antibodies J Mol Biol. 2018 Jul 6;430(14)：2139-2152. doi： 10.1016/j.jmb.2018.05.018. Epub 2018 May 17 PMID：29778602 |
Note on publication | The original publication explores the development of antibodies targeting the host antigen CD81 to block hepatitis C viral entry. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
