Key features and details | |
Cat. No. | MABL-1104 |
Name | Anti-CD74 mAbs |
Clone No. | AFD- LN-2 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, ELISA, FC, IF, IHC |
Species Reactivity | Rat, Human, Mouse |
Basic Information | |
Specificity | LN-2 antibody recognizes CD74. It reacts with an epitope residing within 60 amino acids of the extracytoplasmic, COOH terminus of the protein. CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. |
Alternative Name | Ia antigen; HLA class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; Ii; p33; LN2 |
UniProt | P04233 |
Immunogen | This antibody was raised by immunizing mice with SU-DHL-4 lymphoma cells. |
Application Notes | Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+). This antibody can be used for the identification of B cell lymphoma and leukemias. It also stains activated neoplastic cells in T cell lymphomas and Sternberg-reed cells.Furthermore, this antibody was shown to be capable of distinguishing small cell lung cancer from a non small cell lung cancer as a part of a panel of antibodies (Ioachim et al., 1996). Furthermore, LN2 was successfully used in immunohistochemical and flow cytometric analysis of lung cancer tissue and cells (Ioachim et al., 1996). Finally, this antibody is also useful in immunofluorescence analysis of CD74, as it was very effective in labelling this protein in CD74-transfected COS-7 cells (Leng et al., 2003). |
Antibody First Published | Epstein et al. Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028-36. PMID:6376628 |
Note on publication | This article describes the LN-2 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
