Key features and details | |
Cat. No. | MABL-1103 |
Name | Anti-CD74 mAbs |
Clone No. | AFD- LL1 (EPB1) |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, RIA, WB, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody reacts with the cell surface epitope of the invariant chain (Ii) subunit of the immatrue MHC class-II antigen CD74. |
Alternative Name | Ii; p33; DHLAG; HLADG; Ia-GAMMAHLA; HLA Class II histocompatibility antigen gamma chain; HLA-DR antigens-associated invariant chain; Ia antigen-associated invariant chain; |
UniProt | P04233 |
Immunogen | This antibody was generated by immunizing female BALB/c mice with whole Raji cells or a crude membrane preparation. |
Application Notes | Radioimmunobinding and flow cytometric analysis was used to analyse binding of LL1 to peripheral blood. LL1 did not react with T cell lines nor with normal diploid cells. The reactivity of LL1 (EB- 1) to the tumor cells was checked using a live cell indirect immunoflurescence staining assay. LL1 stained positive for cell lines Raji (maxilla), Daudi (B-lymphoblast), RAMOS (b-lymphocyte), SU-DHL-7, ARH-77 (plasma cell leukemia), HS-Sultan (plasmacytoma). LL1 is reported to recognize most of the small and large lymphocytes of the germinal centre and no reactivity to marginal zone B-cells. It is also reported to react with interdigitating histiocytes of the T-cell zones. It was further reported that LL1 bound positively to Western blotted Raji cell antigens (PMID: 2663143). |
Antibody First Published | Pawlak-Byczkowska et al. Two New Monoclonal Antibodies EPB-1 and EPB-2 Reactive with Human Lymphoma1. Cancer Research (1989); Volume 49; pg 4568-4577. PMID:2663143 |
Note on publication | Describes the generation and immunohistochemical characterization of antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
