Key features and details | |
Cat. No. | MABL-1084 |
Name | Anti-CD70 mAbs |
Clone No. | AFD- BU69 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FACS, IP, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds human CD70, which is the CD27 ligand. This protein is present only on activated dendritic cells and T lymphocytes, and represents a therapeutic target of relevance for autoimmune diseases. |
Alternative Name | TNFRS7; Tumor Necrosis Factor Ligand Superfamily Member 7; CD27 Ligand; CD27LG; TNFSF7; CD27L; Tumor Necrosis Factor Ligand 8A; Surface Antigen CD70; Ki-24 Antigen; CD70 Antigen; TNLG8A; TAN1-7; TAN17 |
UniProt | P32970 |
Immunogen | Murine antibodies were produced by immunising BALB/c mice using the Waldenstrom's macroglobulinaemia cell line WM-1. Hybridomas were generated by fusion of immunised cells with the myeloma cell line P3/NS1/1-Ag4.1 via established methods. |
Application Notes | BU69 has been used for FACS and immunocytochemistry analysis of the cell-surface expression levels of CD70 in renal cell carcinomas (Adam et al, 2006). It was shown in this study that cell- surface binding of BU69 to CD70 caused rapid internalisation of the cell-surface glycoprotein. |
Antibody First Published | Edited by Schlossman et al. Leukocyte typing V: White cell differentiation antigens Oxford |
Note on publication | Describes the repertoire of leukocyte differentiation antigens (i.e. Clusters of Differentiation, and other such cell-surface molecules), specifically using a panel of mAbs to then facilitate characterisation of distinct cell types based on their antibody reactivity patterns. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
