Key features and details | |
Cat. No. | MABL-1080 |
Name | Anti-CD7 mAbs |
Clone No. | AFD- YTH3.2.6 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | ELISA, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds CD7. |
Alternative Name | GP40; LEU-9; TP41; Tp40; T-cell leukemia antigen; P41 protein; YTH3.2.6AG5 |
UniProt | P09564 |
Immunogen | The k-secreting myeloma line Y3/Ag1.2.3 was fused with spleen cells from DA rats immunised with normal human lymphocytes. |
Application Notes | The fraction of human lymphocyte cells recognised by YTH 3.2 was analysed by flow cytometry, using fluorescein-labelled F(ab')2 fragments of rabbit anti-(rat Ig) to stain the antibody. Binding has also been characterised by ELISA. In preliminary tests, graft rejection in patients with steroid-resistant kidney rejection indicated that graft rejection could be reversed by YTH 3.2 injections (Hale et al, 1985). This antibody has been used to stain T-cell dependent areas in immunohistology (King et al, 1989). |
Antibody First Published | Hale et al Therapeutic potential of rat monoclonal antibodies: isotype specificity of antibody-dependent cell-mediated cytotoxicity with human lymphocytes. J Immunol 1985; 134:3056-3061 PMID:3980990 |
Note on publication | Describes the generation and analysis of rat monoclonal antibodies for use in serotherapy. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
