Key features and details | |
Cat. No. | MABL-1078 |
Name | Anti-CD7 mAbs |
Clone No. | AFD- TH69 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | block;, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the extracellular domain of human CD7. CD7 is expressed on the majority of immature and mature T-lymphocytes, and T cell leukemia. It is also found on natural killer cells, a small subpopulation of normal B cells and on malignant B cells. CD7 is useful in the identification of precursor T cells as well as an important marker for the identification of leukemic T cells. |
Alternative Name | GP40; TP41; T-cell antigen CD7; T-cell leukemia antigen; T-cell surface antigen Leu-9; TH-69 |
UniProt | P09564 |
Immunogen | |
Application Notes | The therapeutic application of this antibody was explored to determine its potential use in the treatment of Human T-cell acute lymphocytic leukemia (ALL). When nude mice bearing approx. 2 g of tumor were treated with a single injection of CD7 antibody TH-69, 82.6% reached complete remission within 10 d whereas 13.0% showed partial remission. (PMID: 8904888). This antibody was also used in the development of a recombinant immunotoxin by linking the scFv version of this antibody to a truncated Pseudomonas exotoxin A fragment containing the catalytic domains II and III but lacking the receptor binding domain I. Approximately 20% of leukemic cells from a patient with CD7-positive acute T-cell leukemia kept in long-term primary culture for 30 cell generations were killed within 96 h after treatment with the toxin (PMID: 12019163). This antibody was also used in the generation of a fusion protein scFvCD7:sFasL, that is designed to have leukemia-restricted activity. wherein the scFv version of this antibody is genetically linked to soluble homotrimeric FasL. |
Antibody First Published | Gramatzki et al. Monoclonal antibodies developed for diagnosis of T cell and very immature leukemias. Blut, 50 (1985), p. 216. |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
