Key features and details | |
Cat. No. | MABL-1066 |
Name | Anti-CD64 mAbs |
Clone No. | AFD- H22 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | competition studies, toxicity assays, ELISA, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody specifically targets human CD64, recognizing an epitope distinct from the Fc binding domain. |
Alternative Name | FcγRI; High affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I; Fc-gamma RI; FcRI; Fc-gamma RIA |
UniProt | P12314 |
Immunogen | The original antibody was generated by cloning a single-chain fragment (scFv) derived from the murine anti-human CD64 monoclonal antibody (mAb) clone "m22". Clone m22 was originally described by Guyre et al. (1989). |
Application Notes | This antibody can be bound to a toxin so that it displays specific cytotoxicity and thus can be used to eliminate AML cells selectively. This antibody was bound to a truncated version of the Pseudomonas exotoxin A (ETA'). The binding activity of the VH/VL antibody format was unaffected by the fusion of the m22(scFv) coding regions to the ETA' coding sequences. FC analysis indicated that this immunotoxin drove 41% of primary leukemia cells from a patient with CD64-positive AML into early apoptosis (Tur et al., 2003; PMID: 14679004). Similarly, in an in vivo mouse model, IHC analysis revealed efficient elimination of human CD64+ tumor cells in mouse organs (Tur et al., 2011; PMID: 21077160). |
Antibody First Published | Tur et al., Monoclonal antibodies that bind to distinct epitopes on Fc gamma RI are able to trigger receptor function & Recombinant CD64-Specific Single Chain Immunotoxin Exhibits Specific Cytotoxicity against Acute Myeloid Leukemia Cells Journal of immunology, 1 September 1989, Vol.143(5), pp.1650-5 & CANCER RESEARCH 63, 8414 – 8419, December 1, 2003 PMID:14679004 |
Note on publication | The original article by Tur et al. (2003; PMID: 14679004) described the development of an immunotoxin-conjugated recombinant anti-CD64 monoclonal antibody and demonstrated its efficacy in targeting CD64+ acute myeloid leukemia cells, inducing apoptosis. The parental murine antibody clone "m22", used to generate clone H22, was first reported by Guyre et al. (1989; PMID: 2474608). It was employed in FC, IP, staining, cytotoxicity assays, and cross-blocking analyses to characterize FcγRI epitopes, showing its ability to trigger cytotoxicity. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
