Key features and details | |
Cat. No. | MABL-1036 |
Name | Anti-CD53 mAbs |
Clone No. | AFD- HD77 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the extracytoplasmic part of human CD53, a pan-leukocyte antigen. |
Alternative Name | TSPAN-25; Leukocyte surface antigen CD53; Tetraspanin-25; MOX44; TSPAN25 |
UniProt | P19397 |
Immunogen | This antibody was raised by immunising mice with human leukemia cells. |
Application Notes | This antibody has applications in the detection of normal and neoplastic white blood cells. This antibody has been used to stain tumour and mononuclear cells for CD53 expression in flow cytometric analysis of samples from ovarian carcinoma patients (Marme et al, 2002), and cells of the colon carcinoma line HT29 (Koretz et al, 1992). In immunohistochemical analysis of frozen tissue sections, this antibody has been shown to react with osteoclasts and osteoclast-like giant cells in malignant fibrous histiocytoma and in benign giant cell tumor of tendon sheath (Mechtersheimer et al, 1990). This antibody has been used to re-immunoprecipitate CD53 from PKCα immunoprecipiates (Zhang et al, 2001). |
Antibody First Published | Mechtersheimer et al. Expression of HLA-A,B,C, beta 2-microglobulin (beta 2m), HLA-DR, -DP, -DQ and of HLA-D-associated invariant chain (Ii) in soft-tissue tumors. Int J Cancer. 1990 Nov 15;46(5):813-23. PMID:2228310 |
Note on publication | Describes the original use of this antibody, and its use in immunohistochemical analysis of frozen tissue sections. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
