Key features and details | |
Cat. No. | MABL-1021 |
Name | Anti-CD49F mAbs |
Clone No. | AFD- GoH3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | FC, IF, IHC |
Species Reactivity | Dog, Human, Mouse |
Basic Information | |
Specificity | This antibody is specific for the mouse, human and dog version of the CD49F protein. CD49F is a receptor for laminin on platelets. Furthermore CD49F is present in oocytes and is involved in sperm-egg fusion. |
Alternative Name | Integrin alpha-6; ITGA6; CD49 antigen-like family member F; VLA-6 |
UniProt | P23229 |
Immunogen | This antibody was raised by immunizing a BALB/c rat with amino acids 1242-1255 of the C- terminus of the Human c-erbB-2/HER-2/neu protein |
Application Notes | To determine the influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells, cryo-sections of human cultured gingiva were stained for immunohistochemistry using the mouse version of this antibody. (Becker et al, 2002; pmid：12013557). To isolate pancreatic islet progenitor cells from foetal mice, a mouse pancreatic cell dispersion was used for flow cytometry using the mouse version of this antibody (Sugiyama et al, 2006; pmid：17190805). To present evidence that HPV binds specifically to laminin 5, cultured keratinocytes infected with VLPs from HPV-11, were stained using the mouse version of this antibody (Culp et al, 2006; pmid：16940506). |
Antibody First Published | Becker et al. Influence of anti-CD49f and anti-CD29 monoclonal antibodies on mitotic activity of epithelial cells (HaCaT) and gingival fibroblasts in vitro Eur J Oral Sci. 2002 Apr;110(2)：137-43. PMID：12013557 |
Note on publication | Monoclonal antibodies against the VLA-6 laminin receptor subunit alpha6 interrupted interactions between epithelial cells (HaCaT cells) and their extracellular matrix and thus resulted in reduction of proliferation rates by more than 50%. The same effect was observed with anti alpha1- antibodies. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
