Key features and details | |
Cat. No. | MABL-1017 |
Name | Anti-CD49b mAbs |
Clone No. | AFD- 16B4 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for the α2 integrin subunit, and recognises an epitope outside the von Willebrand factor A-type, or collagen-binding domain. Integrins are heterodimeric integral membrane glycoproteins, which are expressed by a range of cells, including T cells, NK cells, fibroblasts and platelets, where they mediate cell adhesion. |
Alternative Name | Integrin alpha-2; CD49 antigen-like family member B; Collagen receptor; Platelet membrane glycoprotein Ia; GPIa; VLA-2 subunit alpha; alpha 2 subunit of VLA-2 receptor; BR; CD49B; human platelet alloantigen system 5; ITGA2; platelet antigen Br; platelet glycoprotein GPIa; very late activation protein 2 receptor, alpha-2 subunit |
UniProt | P17301 |
Immunogen | |
Application Notes | This antibody has been used in flow cytometric analysis to investigate the surface expression of α2 integrin by cultured human endothelial cells (Sincock et al, 1999), and in immunoprecipitation analysis to assess the interaction between PETA-3 and integrins on the surface of endothelial cells (Sincock et al, 1999). Alexa Fluor 647-conjugated 16B4 has been used to evaluate the expression of integrin α2β1 on the surface of platelets by confocal imaging (Poulter et al, 2017). This antibody has been employed to detect integrin α2β1 expression through in-cell Western blotting analysis in primary fibroblasts (Suarez et al, 2014). This antibody has also been used to coat polystyrene beads as part of a microsphere-based cellular adhesion assay; human osteosarcoma cells transfected with the integrin a2 subunit displayed high levels of interaction with beads coated in this anti-α2 integrin antibody (Connors & Heino, 2004). |
Antibody First Published | Sincock et al. PETA-3/CD151, a member of the transmembrane 4 superfamily, is localised to the plasma membrane and endocytic system of endothelial cells, associates with multiple integrins and modulates cell function Journal of Cell Science 112, 833-844 (1999) PMID：10036233 |
Note on publication | Describes the original use of this antibody in flow cytometric and immunoprecipitation analysis. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
