Key features and details | |
Cat. No. | MABL-1010 |
Name | Anti-CD47 mAbs |
Clone No. | AFD- mIAP301 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | IP, WB, Block, FC, IF |
Species Reactivity | Mouse |
Basic Information | |
Specificity | MIAP301 binds specifically to the extracellular domain of CD47 (exon 2 that encodes both the predicted signal peptide clevage site and the entire Ig domain). CD47 plays a role in cell adhesion by acting as an adhesion receptor for THBS1 on platelets and in (beta-3) integrin modulation. Functions as a SIRPA receptor - binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. CD47 also interacts with SIRPG which mediates cell-cell adhesion, enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. Plays an important role in memory formation and synaptic plasticity in the hippocampus. |
Alternative Name | IAP; Leukocyte surface antigen CD47; Integrin-associated protein; integrin associated protein; MIAP 301; MIAP-301 |
UniProt | Q61735 |
Immunogen | MIAP301 was prepared by immunizing rats with intact CD47 purified from mouse placenta. |
Application Notes | MIAP301 can block murine CD47 interactions with SIRPα and functionally blocks CD47 in vivo in murine soft tissue ischemia and skin graft models (Soto-Pantoja et al. 2012). This antibody has also been used for FC (Olsson et al. Blood. 2005 May 1; 105(9): 3577–3582.). MIAP301 has also been shown to block KCl-evoked glutamate release from the hippocampus in mice, inhibiting glutamate release and thus impair memory retention (Chang et al. 2001). |
Antibody First Published | Lindberg et al. Decreased Resistance to Bacterial Infection and Granulocyte Defects in lAP-Deficient Mice Science. 1996 Nov 1;274(5288):795-8. PMID:8864123 |
Note on publication | Describes the use of MIAP301 in determining CD47 tissue distribution and to ensure no functional fragment of CD47 was expressed in CD47 -/- KO mice. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
