Key features and details | |
Cat. No. | MABL-1005 |
Name | Anti-CD47 mAbs |
Clone No. | AFD- C47B227 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | crystallography, inhibition |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CD47. The antibody binds a conformational epitope composed of residues in the C’C’’ loop (L54) and B-strand C*’ (K56 and T58) regions. |
Alternative Name | IAP; MER6; OV-3; Leukocyte surface antigen CD47; Antigenic surface determinant protein OA3; Integrin-associated protein; Protein MER6; ovarian cancer marker |
UniProt | Q08722 |
Immunogen | Three de novo phage libraries displaying Fab variants on phage coat protein pIX were panned against biotinylated human CD47. |
Application Notes | The structure of the Fab fragment in complex with CD47 was determined. The binding affinity of the antibody to human and cyno CD47 were measured (Kd= 1nM and 0,5 nM respectively). The antibody inhibited CD47 from interacting with SIRP alpha (US10864268). |
Antibody First Published | |
Note on publication | |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
