Anti-CD47 mAbs

Cat. No.

MABL-1000

Name

Anti-CD47 mAbs

Clone No.

AFD- 105D11

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Block, ELISA, FC

Species Reactivity

Human, Cynomolgus Monkey

Basic Information

Specificity

This antibody binds the extracellular domain of the human CD47 and cross reacts with cynomolgus monkey CD47. CD47 is   broadly distributed on normal adult tissues, as well as ovarian tumors, and   is especially abundant in some epithelia and the brain. IAP or integrin   associated protein has a role in both cell adhesion by acting as an adhesion   receptor for THBS1 on platelets, and in the modulation of integrins. It   further plays an important role in memory formation and synaptic plasticity   in the hippocampus. It also acts as a receptor for SIRPA. Binding to SIRPA   prevents maturation of immature dendritic cells and inhibits cytokine   production by mature dendritic cells.

Alternative Name

IAP; MER6; OV-3; Leukocyte surface antigen   CD47; Antigenic surface determinant protein OA3; Integrin-associated protein;   Protein MER6; ovarian cancer marker

UniProt

Q08722

Immunogen

The original antibody was generated by immunizing BALB/c mice with recombinant human CD47-mFc fusion protein.

Application Notes

The binding characterization of this   antibody to recombinant human Cd47-mFc fusion protein was done using ELISA.   This antibody could also bind human and cynomolgus monkey CD47 expressed on   the surface of cells in a flow cytometric assay. This antibody was also   reported to block the binding between SIRPα-hFc protein and human CD47   expressed on the cell surface (CN110724672).

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.