Key features and details | |
Cat. No. | MABL-979 |
Name | Anti-CD45 mAbs |
Clone No. | AFD- BC8 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | in vivo, therapeutic, FC |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific for CD45, binding to all CD45 isoforms. CD45 is a 180-240kD glycoprotein member of the protein tyrosine phosphatase (PTP) family known for its involvement in regulating a variety of cellular processes, including cell growth, differentiation, mitotic cycle, and oncogenic transformation. |
Alternative Name | Receptor-type tyrosine-protein phosphatase C; L-CA; Leukocyte common antigen; T200; Lymphocyte antigen 5 |
UniProt | P08575 |
Immunogen | |
Application Notes | The antibody was used to target human Ramos lymphoma xenografts in athymic mice. The antibody was able to deliver radioiodine to tumors, causing minimal toxicity to normal organs. Further, therapy experiments demonstrated that 90Y-(DOTA)–biotin with BC8-SA cured the mice in a dose dependent manner, and with complete remission of the antibody (Pagel et al., 2003; PMID: 12446461). In vivo studies on Macaca nemestrine revealed the 131l-labeled IgG1 antibody could deliver radiation with relative specificity to lymphohematopoietic tissues. The antibody displayed an association constant (avidity) of 6 x 10^7 (L/mol) for macaque cells and 5 x 10^8 (L/mol) for human cells (Matthews et al., 1991; PMID: 1832994). Furter, 211At labeled BC8-B10 is of interest for clinical trials involving allogeneic hematopoietic transplantation in the treatment of advanced hematological malignancies (Li et al., 2018; PMID: 30335787). |
Antibody First Published | Matthews et al. Radiolabeled anti-CD45 monoclonal antibodies target lymphohematopoietic tissue in the macaque Blood. 1991 Oct 1;78(7):1864-74. PMID:1832994 |
Note on publication | The original paper describes the reactivity of the 131I-labeled antibody with the CD45 antigen in Macaca nemestrina. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
