Key features and details | |
Cat. No. | MABL-968 |
Name | Anti-CD44 mAbs |
Clone No. | AFD- Hermes-3 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | Blocking, ICC, Western blot, ELISA, FC, IF, IHC |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody is specific for CD44 (gp90 epitope cluster III). Hermes-3 recognizes Hermes antigen homologues on monkey, goat, and sheep lymphocytes. |
Alternative Name | CDw44; Epican; Extracellular matrix receptor III; ECMR-III; GP90 lymphocyte homing/adhesion receptor; HUTCH-I; Heparan sulfate proteoglycan; Hermes antigen; Hyaluronate receptor; Phagocytic glycoprotein 1; PGP-1; Phagocytic glycoprotein I; CD44/H-CAM antibody; Hermes 3 |
UniProt | P16070 |
Immunogen | This antibody was produced by immunizing BALB/c mice with purified Hermes-1 antigen from KCA, a human B lymphoblastoid line that binds preferentially to mucosal HEV. |
Application Notes | This antibody has been used in IF, IHC (immunoperoxidase method), cross-blocking and WB studies to characterise the Hermes-defined antigens (Picker, 1989). This antibody is able to block lymphocyte binding to mucosal HEV, without affecting binding to HEV in peripheral lymph nodes and synovium (Jalkanen, 1987). H3-51 is cross-blocked by this antibody, indicating that the epitopes recognized by these two antibodies overlap (Picker, 1989). |
Antibody First Published | Jalkanen et al. Lymphocyte recognition of high endothelium: antibodies to distinct epitopes of an 85-95-kD glycoprotein antigen differentially inhibit lymphocyte binding to lymph node, mucosal, or synovial endothelial cells The Journal of Cell Biology, 1987, Vol.105(2), p.983-990 PMID:2442176 |
Note on publication | Describes the original use of this antibody, along with others, to characterise the molecules involved of lymphocyte recognition of HEV. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
