Cat. No.
MABL-2149
Application
inhibit, IP, Block, FC
Isotype
Engineer antibody
Species Reactivity
Guinea Pig, Human
Clone No.
HP1/2
From
Recombinant Antibody
Specificity
This antibody specifically binds epitope B of the alpha 4 chain of human integrin alpha 4 (CD49d). Integrins alpha-4/beta-1 (VLA-4) acts as a receptor for fibronectin and VCAM1. VLA-4 is atypical because it is mostly restricted to lymphoid and myeloid cells, and indirect evidence had suggested that it might be involved in various cell-cell interactions. In addition, VLA-4 has been shown to mediate T and B lymphocyte attachment to the heparin II binding fragment of human plasma fibronectin (FN). This antibody is also reported to cross react with integrin alpha-4/beta-7 (α4β7).
Alternative Names
CD49d; VLA-4; α4; VLA4; ITGA4; α4β1; α4 integrin; VLA4 heterodimer (α4β1); Integrin alpha-4; Integrin alpha-IV; VLA-4 subunit alpha; CD49 antigen-like family member D; B1 integrin very late activation antigen-4
UniProt
P13612
Immunogen
Application Notes
This antibody can be used for the immunoprecipitation of VLA-4 from cell lysates (PMID: 2430809). This antibody is also capable of detecting integrin alpha 4 expressed on the surface of Ramos cells by flow cytometry (PMID: 2430809; 1689216). This antibody can also block adherence of CTT leukocytes to recombinant soluble VCAM 1-coated plates in vitro (PMID: 7686922). This antibody can also block the interaction of VLA-4 with fibronectin. This antibody does not cause cell aggregation (PMID: 1709929). This antibody can bind soluble integrin alpha-4 in an ELISA. This antibody also inhibited the binding of PAF-stimulated eosinophils to MAdCAM-1 transfectants. This antibody also inhibited binding of unstimulated eosinophils to soluble recombinant human (rh)VCAM-1 and fibronectin (Fn) (PMID: 8911148).
Antibody First Published
Sánchez-Madrid et al. VLA-3: a novel polypeptide association within the VLA molecular complex: cell distribution and biochemical characterization. Eur J Immunol. 1986 Nov;16(11):1343-9. PMID:2430809
Note on publication
Describes the generation of eight antibodies against VLA-4 antigen.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
