Cat. No.
MABL-2147
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Mouse
Clone No.
193020
From
Recombinant Antibody
Specificity
This antibody is specific for mouse insulin 2.
Alternative Names
Insulin
UniProt
P01326
Immunogen
The original antibody was generated by fusing spleen cells from two different NOD mice with recent onset diabetes with the mouse myeloma cell line NS-1.
Application Notes
The activity of this anti-insulin autoantibody within supernatants was evaluated using ELISA (Pléau et al., 1993; PMID: 8413326).
Antibody First Published
Pléau et al. Evidence for antigen driven selection in two monoclonal auto- antibodies derived from nonobese diabetic mice Mol Immunol. 1993 Oct;30(14):1257-64. doi: 10.1016/0161- 5890(93)90041-9 PMID:8413326
Note on publication
The original publication discusses the creation and characterization of two monoclonal autoantibodies directed against insulin and peripherin.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
