Anti-CD40 ligand mAbs

Cat. No.

MABL-950

Name

Anti-CD40 ligand mAbs

Clone No.

AFD- 5c8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

catalysis, crystallography, in vivo, IP, FC

Species Reactivity

Human

Basic Information

Specificity

 This antibody is specific for human CD40   ligand.

Alternative Name

CD40LG; TRAP; CD154; CD40 ligand; CD40L;   TNFSF5, tumor necrosis factor ligand superfamily member 5; tumor necrosis   factor related activation protein

UniProt

 P29965

Immunogen

 The original antibody was raised by immunizing   a BALB/c mouse with D1.1 cells.

Application Notes

The original version of this antibody was   used to catalyse disfavoured endo-tet cyclization reactions of trans-epoxy   alcohols (Gruber et al., 1999; PMID:10353817). The structure of the fab   version of the antibody was determined using x-ray crystallography (Gruber et   al., 1999; PMID:10353817, Karpusas et al., 2001; PMID:11525169). The original   antibody was used for flow cytometry on D1.1 cells. It only showed binding to   D1.1 cells but not to B2.7 cells. This antibody was used for   immunoprecipitation on Jurkat cell lysates and precipitated a 30kDa protein.   This antibody also shows the ability to inhibit D1.1 cell induced B cell   activation (Lederman et al., 1992; PMID:1348081). The half life of this   antibody in cynomolgus monkeys was measured.

Antibody First Published

Lederman et al. Identification of a novel   surface protein on activated CD4+ T cells that induces contact-dependent B   cell differentiation (help) J Exp Med. 1992 Apr 1;175(4):1091-101.   PMID:1348081

Note on publication

To identify T cell surface molecules that   mediate contact-dependent T helper function, the original paper generated a   monoclonal antibody (mAb), designated 5c8, that inhibits D1.1- mediated B   cell activation and immunoprecipitates a novel 30-kD protein structure from   surface-iodinated D1.1 cells.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.