Key features and details | |
Cat. No. | MABL-945 |
Name | Anti-CD40 mAbs |
Clone No. | AFD- chi220 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | therapeutic, Block |
Species Reactivity | Human |
Basic Information | |
Specificity | This antibody binds the CD40 and blocks the humoral immune responses to T cell dependent antigens in a dose dependent manner. |
Alternative Name | TNFSF5; CDw40; TRAP; p50; Bp50; CD40L receptor; Tumor necrosis factor ligand superfamily member 5; B-cell surface antigen CD40; T-cell antigen Gp39; TNF-related activation protein. |
UniProt | P29965 |
Immunogen | This antibody was generated by immunizing female BALB/c mice with a fusion protein containing extracellular domain of human CD40 fused to the hinge CH2 and CH3 domains of a murine IgG2b antibody. |
Application Notes | This antibody blocks the interaction between CD40 and gp39. Chi220 was used either alone or in combination with CTLA4-Ig in an immunosuppresive therapy study to check the survival of renal allografts in non human primate models (PMID: 12394833). Chi220 blocks binding of CD154, it also has partial agonist properties and weak stimulatory potential. It synergisticaly works with LEA29Y (belatacept) to prolong the islet allograft survival in a non-human primate Macaca fascicularis (PMID: 15611281). A study on protection of islets xenographs in diabetic macaques used a combination of Chi220 with IL-2 receptor specific antibody Basiliximab for immunosuppression with induction therapy. Chi220 is used as an sCD154 blocking agent and it was seen that it binds sCD154 in a dose dependant manner (PMID: 21920020). |
Antibody First Published | Adams et al. Development of a chimeric anti-CD40 monoclonal antibody that synergizes with LEA29Y to prolong islet allograft survival. J Immunol. (2005); 174(1):542-50.PMID:15611281 |
Note on publication | Describes the generation and characterization of this antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
