Key features and details | |
Cat. No. | MABL-939 |
Name | Anti-CD40 mAbs |
Clone No. | AFD- 2C5 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | agonist, immune activation, FC, IF |
Species Reactivity | Chicken |
Basic Information | |
Specificity | This antibody recognises extracellular domain of chicken CD40. CD40, an integral membrane glycoprotein of the TNF-receptor superfamily, is expressed mainly on professional antigen-presenting cells (APCs), including B-cells, macrophages, and dendritic cells. However, high levels of CD40 can also be detected on a wide range of tumor cells of the bladder, breast, and ovary. |
Alternative Name | CD40L receptor; CDw40; Bp50; B-cell surface antigen CD40; Tumor necrosis factor receptor superfamily member 5 |
UniProt | |
Immunogen | This antibody was raised by immunising mice with a recombinant protein corresponding to the extracellular domain of chicken CD40 (chCD40ED). |
Application Notes | 2C5 antibody is a CD40 agonist and can be used to induce immune activation. Agonistic monoclonal anti-CD40 antibodies (mAbs) mimic the effects of CD40L on APCs, leading to enhanced T-cell priming andexpansion, increased antibody production and class switching. This antibody was shown to be a powerful adjuvant eliciting a strong IgG-response in chicken what makes it an important agent in vaccines development (Chen et al., 2012). Furthermore, 2C5 was also demonstrated to be effective in detecting CD40 via immunofluorescence as well as via flow cytometry in chicken B cells and macrophages (Chen et al., 2010). Finally, this antibody was observed to activate NO synthesis in HD11 macrophages, and stimulate DT40 B-cellproliferation (Chent et al., 2010). |
Antibody First Published | Chen et al. Production and characterization of agonistic monoclonal antibodies against chicken CD40. Dev Comp Immunol. 2010 Nov;34(11):1139-43. doi: 10.1016/j.dci.2010.06.014. Epub 2010 Jul 3. PMID:20599554 |
Note on publication | This article describes the generation and characterisation of 2C5 antibody. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
