Anti-CD4, Cytoplasmic tail mAbs

Cat. No.

MABL-937

Name

Anti-CD4, Cytoplasmic tail mAbs

Clone No.

AFD- 10C12

From

Recombinant Antibody

Isotype

Engineer antibody

Application

 Blocking, WB, ELISA, IF

Species Reactivity

Human

Basic Information

Specificity

This antibody recognises LSEKKTCQCPHRFQKTCSPI   peptide of human CD4 epitope within amino acids 414 and 433 of the   cytoplasmic tail region. CD4 is a membrane glycoprotein of T lymphocytes that   interacts with major histocompatibility complex class II antigenes and is   also a receptor for the human immunodeficiency virus.The cytoplasmic tail   region transmits a signal in response to p56-lck. HIV nef is also known to   interact with the cytoplasmic tail of CD4.

Alternative Name

 Leu3/T4; 024; CD4 cytoplasmic tail; T-cell   surface glycoprotein CD4; T- cell surface antigen T4/Leu-3

UniProt

 P01730

Immunogen

 This antibody was raised against a multiple   antigenic peptide; a tetramer linked through a lysine backbone.

Application Notes

10C12 antibody reacts with a poorely studied   tail region of human CD4 and might be used to conduct reasearch on the role   of this fragment of the protein. Furthermore, this antibody can be used for   studies on expression of CD4. One group utilised 10C12to perform Western blot   analysis of CD4 expression in cerebellar granule neurons when researching   interleukin-16 induced c-Fos expression (Fenster et al., 2010). This antibody   may be also used as an effective blocking agent inhibiting Il-16 (Atanackovic   et al., 2012). Finally, 10C12 has also been recommended for studies of the   role of selenium metabolism and selenoproteins in diseases, such as   infectious diseases (HIV) and cancer (Ware, 2003).

Antibody First Published

Ware Gene regulation and overlooked coding   regions : the case for translation past the 3'-UGA codon in CD4. Ph.D.   Thesis. University of Georgia, College of Pharmacy.(2003)

Note on publication

This thesis describes the generation and   characterisation of TL2.1 antibody

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.