Key features and details | |
Cat. No. | MABL-930 |
Name | Anti-CD4 mAbs |
Clone No. | AFD- OX-35 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | functional, IP, WB, ELISA, IF, IHC |
Species Reactivity | Rat |
Basic Information | |
Specificity | OX-35 shows specificity towards rat CD4 antigen. W3/25 and OX-36 bind to different epitopes on CD4 antigen. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction and is involved in regulation of T-cell activation. |
Alternative Name | Leu3/T4; L3T4; T4; Leu3; Leu-3; Ly4; Ly-4; W3/25 antigen; OX35; MRC OX-35; MRC OX35 |
UniProt | P05540 |
Immunogen | OX-35 was prepared by immunizing mice with T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen. |
Application Notes | This antibody is able to label cells expressing CD4 on their surface and can precipitate CD4 from detergent extracts of radioactively labelled thymocytes. |
Antibody First Published | Robinson et al. Macrophage heterogeneity in the rat as delineated by two monoclonal antibodies MRC OX-41 and MRC OX-42, the latter recognizing complement receptor type 3 Immunology 1986 57 239-247 PMID:3512425 |
Note on publication | Describes the original use of this antibody to detect SIRP alpha in labelling, immunoperoxidase and immunofluorescence studies to investigate macrophage heterogeneity. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
