Cat. No.
MABL-1850
Application
functional assay, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
HYB3
From
Recombinant Antibody
Specificity
This antibody is specific for human HLA-A1:MAGE-A1 complex.
Alternative Names
human leukocyte antigen (HLA)/peptide complex HLA-A1·MAGE-A1; tumor-associated HLA-A1·melanoma-associated antigen (MAGE)-A1 complex
UniProt
Immunogen
This antibody was raised by panning against the L chain-shuffling library raised by cloning the G8 VH gene into a vector containing a library of human Ab kappa and lambda L chains.
Application Notes
The structure of the fab version of this antibody in complex with HLA-A1 and MAGE-A1 was eludicated using X-ray crystallography. This was done to determine how the antibody binds to its ligand (Hülsmeyer et al, 2005; pmid:15537658). To characterize this antibody flow cytometry was preformed. Flow cytometry was preformed with the fab version of this antibody on dendritic cells (Chames et al, 2002; pmid:12097420). To assess the expression of low and high affinity scFv-y CD28 and scFv- receptors on gene-transduced primary human T lymphocytes. Flow cytometry was preformed on gene-transduced primary human T lymphocytes using the scFv version of this antibody. Furthermore a cytotoxic assay was preformed on scFv-y + CD28 or scFv-transduced human T lymphocytes. The cytotoxic assay was preformed using Cr51 release assay (Willemsen et al, 2005; pmid:15944290). To characterize this antibody after expressing the antibody in E.coli. An ELISA was preformed using the fab version of this antibody on a biotinylated M1/A1 complex. Furthermore to asses the ability of this antibody to bind M1/A1-positive cells. Flow cytometry was preformed on these cells using the scFv version of this antibody (Saeed et al, 2016; pmid:27022262).
Antibody First Published
Willemsen et al. A major histocompatibility complex-peptide-restricted antibody and t cell receptor molecules recognize their target by distinct binding modes: crystal structure of human leukocyte antigen (HLA)-A1-MAGE-A1 in complex with FAB-HYB3. J Immunol. 2005 Jun 15;174(12):7853-8. PMID:15944290
Note on publication
It was analyzed whether linking the CD28 costimulation structure to it (gamma + CD28) could affect the levels of MHC-restricted cytolysis and/or cytokine production.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
