Cat. No.
MABL-1840
Application
neutralize, ELISA
Isotype
Engineer antibody
Species Reactivity
HIV-1
Clone No.
F11.2.32
From
Recombinant Antibody
Specificity
F11.2.32 Fab binds to HIV-1 PR with a dissociation constant of 5 nM (determined by SPR at pH5.2) at an epitope consisting of MSLPGRWKPKM (aa 36-46). HIV-1 PR is a homodimeric apartate protease that specifically cleaves the viral Gag and Gag/Pol polyprotein precursor. A correlation has been observed between the activity of the protease and the degree of infectivity, and so this protein is a major antiviral target.
Alternative Names
HIV-1 PR; HIV1 PR; HIV1 protease
UniProt
O90777
Immunogen
F11.2.32 was prepared by generating a hybridoma cell line in which myeloma cells were fused with splenocytes derived from BALB/c mice immunized with HIV-1 PR in the presence of Freund's adjuvant. F11.2.32 was selected for its ability to bind to HIV-1 PR by ELISA.
Application Notes
The IgG and Fab variants of F11.2.32 are both able to strongly inhibit the catalytic activity of HIV-1 PR (IC50 ~35 nM). Inhibition occurs by distorting the native structure of the HIV-1 PR at the base of the flap region, which plays an important role in regulating access to the active site. This antibody is also able to detect HIV-1 PR by ELISA.
Antibody First Published
Lescar et al. Preliminary crystallographic studies of an anti-HIV- 1 protease antibody that inhibits enzyme activity Protein Sci. 1996 May;5(5):966-8. PMID:8732768
Note on publication
Describes the generation of the mAb F11.2.32 and its crystallization in complex with peptide fragments of HIV-1 PR.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
