Cat. No.
MABL-1837
Application
crystallization, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1A9D7
From
Recombinant Antibody
Specificity
The antibody specifically recognizes simultaneous acetylations at K5 and K8 of histone H4. Lysine acetylation of core histones is one of the major posttranslational modifications involved in the control of eukaryotic gene expression.
Alternative Names
H4K5acK8ac; Histone H4 protein;
UniProt
P62805
Immunogen
The original antibody was generated by immunizing mice with Histone H4 protein with site- specific acetylations at K5 and K8.
Application Notes
The specificity of the original format of the antibody for histone H4 proteins containing di-acetylation of K5acK8ac was confirmed by ELISA analysis. The antibody detected the histone H4 proteins containing at least the di-acetylation of K5acK8ac western blot analysis. The crystal structure of the complex of di-acetylated histone H4 and 1A9D7 Fab fragment was determined (Handoko et al., 2018; PMID: 30080437).
Antibody First Published
Handoko et al. JQ1 affects BRD2-dependent and independent transcription regulation without disrupting H4-hyperacetylated chromatin states. Epigenetics. 2018;13(4):410-431. PMID:30080437
Note on publication
The paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
