Cat. No.
MABL-1827
Application
Blocking, IB, IP
Isotype
Engineer antibody
Species Reactivity
Bovine, Human
Clone No.
IR 47-46
From
Recombinant Antibody
Specificity
This antibody reacts with human INSR, recognising an epitope on the alpha subunit (amino acids 485-592). It does not cross-react with rat or mouse insulin receptors, and does cross-react with bovine and rabbit receptors.
Alternative Names
CD220; human insulin receptor; insulin receptor; HHF5; IR; 47 46
UniProt
P06213
Immunogen
This antibody was raised by immunising BALB/c mice with IM-9 lymphocytes, and boosting with purified human placental insulin receptor prior to fusion.
Application Notes
This antibody has been used in immunoprecipitation assays with radiolabelled human insulin receptor (hINSR) (Soos et al, 1986). It has also been used in immunoblot analysis with purified hINSR (Soos et al, 1986), revealing that it binds to the receptor’s alpha subunit. This antibody has been shown to cause >80% inhibition of insulin binding to IM-9 cells (Soos et al, 1986), and to block receptor activation.
Antibody First Published
Soos et al. Monoclonal antibodies reacting with multiple epitopes on the human insulin receptor. Biochem J. 1986 Apr 1; 235(1)： 199–208. PMID：2427071
Note on publication
Describes the original generation of this antibody, and the characterisation of its specificity.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
