Cat. No.
MABL-1810
Application
functional assay, WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
AN-LP1
From
Recombinant Antibody
Specificity
This antibody is specific for amino acids 'DTHFPICIFCCGCCHRSKCGMCCKT' of human hepcidin- 25 and does not cross react with murine hepcidin-25. Hepcidin is a an iron-regulating peptide hormone made in the liver. Hepcidin is a cationic, cysteine-rich and tightly folded peptide stabilized by 4 disulfide bonds that plays a major role in innate immunity and iron homeostasis. It controls the delivery of iron to blood plasma from intestinal cells absorbing iron, from erythrocyte-recycling macrophages, and from iron- storing hepatocytes. Hepcidin acts by binding to and inactivating the sole cellular iron exporter, ferroprotein, which delivers iron to plasma from all iron-transporting cells.
Alternative Names
HAMP; hHepc; HEPC; LEAP1; LEAP-1; Hepcidin; Liver-expressed antimicrobial peptide 1; CNCM I-3794
UniProt
P81172
Immunogen
The original antibody was generated by immunizing mice with human hepcidin peptide 'DTHFPICIFCCGCCHRSKCGMCCKT'.
Application Notes
The original mouse IgG1 antibody binds human hepcidin-25 with a binding affinity of Kd= 99 pM. The binding characterization of this antibody to synthetic human hepcidin-25 was done using ELISA. This antibody can be used for the identification of synthetic human hepcidin or human hepcidin from the sera of patients with an inflammatory disease using dot blot and western blot. This antibody was also used for the immunohistochemical analysis of paraffine-embedded human liver biopsies. The biological activity of this antibody was tested by incubating this antibody with macrophages from J774 murine cell line, and it was reported that this antibody induces ferroprotein degradation (US8487081).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
