Cat. No.
MABL-1795
Application
neutralize
Isotype
Engineer antibody
Species Reactivity
Anhui, HK/156, Indo), Influenza virus H5N1 (VN/1203
Clone No.
H5.3
From
Recombinant Antibody
Specificity
H5.3 binds to H5 at the head domain at an epitope which involves the 190 loop. The epitope was determined to be LGVSSA (aa 134-139) and YQGKSS (aa 142-147) of the head domain by DXMS (deuterium exchange mass spectrometry). This antibody shows reactivity with the wild-type H5N1 field strains VN/1203 (EC50 ~ 17 ng/ml), HK/156 (EC50 ~ 18 ng/ml), Anhui (EC50 ~ 2.8 ug/ml) and Indo (EC50 ~ 10 ug/ml). HA is a viral glycoprotein initially assembled as a trimer on the viral envelope as the preprotein HA0. It is subsequently cleaved by host proteases.
Alternative Names
H5 HA
UniProt
Q5EP31
Immunogen
H5.3 was generated from hybridomas created by fusing PBMCs (peripheral blood mononuclear cells) from human donors who had been previously received an A/Vietnam/1203/2004 H5N1 subunit vaccine with myeloma cells. The hybridoma cells were screened for those showing binding affinity for H5 HA.
Application Notes
H5.3 binds to H5 where it is able to block the receptor binding site on HA by inserting its CDR3 into the site (IC50 ~ 20 ng/ml). H5.3 can neutralize wild-type VN/1203, rdt VN/1203 K and VN/1203 F pseudovirions, all with IC50s of 5 ng/ml. This antibody also has potent HI (hemagglutination inhibition) activity (80 ng/ml).
Antibody First Published
Thornburg et al. Human antibodies that neutralize respiratory droplet transmissible H5N1 influenza viruses. J Clin Invest. 2013 Oct;123(10):4405-9. PMID:23999429
Note on publication
Describes the generation of anti-H5 HA antibodies. Their binding and neutralizing activities were studied in vitro. The structure of H5.3 was determined by x-ray crystallography and a low- resolution structure of H5.3 complexed with VN/1203 HA trimer was determined by EM.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
