Cat. No.
MABL-1758
Application
functional assay, immunofluorescence
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
2E12LI
From
Recombinant Antibody
Specificity
This antibody is capable of recognizing histone H3 proteins with a trimethylation at Lys27. It specifically recognizes and binds trimethyl lysine and amino acids surrounding it comprising KQLATKAAR(trimethyl K)SAPATGGVKC. Methylation is a type of post-translational modification (PTM) which is caused by covalent addition of methyl group to proteins, DNA or other molecules. PTMs have fundamental roles in chromatin structure and function. The PTMs that have taken place in histone proteins can affect gene expression by altering chromatin structure. Histone modifications have also been known as epigenetic markers. Defects in the PTMs pathway have been associated with the occurrence and progression of various human diseases, such as cancer, heart failure, autoimmune diseases, and neurodegenerative disorders such as Parkinson's disease, Alzheimer's disease, and Huntington's disease.
Alternative Names
Trimethylated Histone H3 Lys27; trimethyl-Histone H3 (Lys27); histone H3; H3K27; Trimethylated histone H3; Histone H3 trimethylated at Lysine 27; nucleosome; H3K27ME3; post translational modification; histone post-translational modification; 2E12 mutant M86L/M158I; 2E12
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with a 20-mer peptide (KQLATKAAR (trimethyl K) SAPATGGVKC) containing histone H3K27 trimethylated lysine and conjugated with KLH.
Application Notes
This antibody was used for the development of a H3K27me3‐specific intracellular antibody or H3K27me3‐mintbody, which was a fusion protein comprising variable domain regions of 2E12LI antibody fused to a fluorescent protein and expressed in mouse teratocarcinoma cell line MC12. This mintbody was capable of tracking H3K27me3 in living cells. Mouse MC12 cells, which stably express H3K27me3‐mintbody (sfGFP) was used to localize H3K27me3‐enriched chromatin in living cells. The binding specificity of bacterially expressed and purified 2E12LI‐sfGFP in vitro was evaluated using a modified histone peptide array. (PMID: 33605056).
Antibody First Published
Jian Xu et al. H4K20me1 and H3K27me3 are concurrently loaded onto the inactive X chromosome but dispensable for inducing gene silencing. EMBO Rep. 2021 Mar 3; 22(3): e51989. PMID:33605056
Note on publication
Describes the generation of an H3K27me3‐specific intracellular antibody for localization of histone PTMs.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
