Cat. No.
MABL-1734
Application
therapeutic, WB, ELISA
Isotype
Engineer antibody
Species Reactivity
Cryptosporidium parvum
Clone No.
4H9
From
Recombinant Antibody
Specificity
This antibody binds the GP25-200 antigen of Cryptosporidium parvum. Cryptosporidium parvum is a coccidian parasite that infects intestinal epithelium and causes diarrheal disease in humans and other agriculturally important food animals for which specific therapy and immunoprophylaxis are unavailable. Cryptosporidiosis is self-limiting in hosts with normal immune systems; however, in neonates, the elderly, and hosts having congenital or acquired immunodeficiency diseases or chemotherapy-induced immunosuppression, cryptosporidial enterocolitis may become chronic and have severe consequences.
Alternative Names
GP25-200 antigen
UniProt
Immunogen
The original antibody was generated by immunizing female BALB/c mice intraperitoneally and subcutaneously with purified P23 emulsified in monophosphoryl A-trehalose dimycolate adjuvant.
Application Notes
This antibody can be used for the identification of sporozoites and GP25-200 antigen of C. parvum using western blot. The identification of sporozoite-neutralizing antibody was done using ELISA (PMID: 10768951). This antibody in combination with PLA2 or LL37 was also used in the generation of a biocide fusion proteins to kill C. parvum sporozoites in vitro and in vivo (PMID: 20086143).
Antibody First Published
Schaefer et al. Characterization and formulation of multiple epitope-specific neutralizing monoclonal antibodies for passive immunization against cryptosporidiosis. Infect Immun. 2000 May;68(5):2608-16. PMID:10768951
Note on publication
Describes the generation and characterization of antibodies against multiple epitope of C. parvum to provide passive immunization against Cryptosporidiosis.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
