Anti-CD3e mAbs

Cat. No.

MABL-918

Name

Anti-CD3e mAbs

Clone No.

AFD- 33H1

From

Recombinant Antibody

Isotype

Engineer antibody

Application

WB, ELISA, IHC

Species Reactivity

Human

Basic Information

Specificity

This antibody binds human CD3e. It is part   of TCR-CD3 complex present on T-lymphocyte cell surface that plays an   essential role in adaptive immune response. It also plays a role in signal   transduction during T cell development and in correct T-cell development.

Alternative Name

T3E; T-cell surface glycoprotein CD3 epsilon   chain; T-cell surface antigen T3/Leu-4 epsilon chain; CD3-ε; CGMCC NO 21980;   SP7

UniProt

P07766

Immunogen

The original antibody was generated by   immunizing BALB/c mice with recombinant human CD3 antigen (amino acids   23-126).

Application Notes

This binding specificity of this antibody for   human CD3e was checked using ELISA. The affinity constant of the original  IgG2a antibody was determined to be 1.92×10^9 M^(-1). This antibody can   recognize human CD3e recombinant protein in an immunoblot assay. This   antibody is similar to commercially available anti-CD3 antibody SP7 in   immunohistochemical staining of T cell lymphomas. This antibody can also be   used for the immunohistochemical staining of normal tissues like brain,   heart, cerebellum, esophageal, adrenal, stomach, ovary, small intestine, pancreatic,   colorectal, paratony gland, liver, pituitary, salivary gland, testicular,   kidney, thyroid, prostate, breast, uterus, spleen , Bladder, tonsil, skeletal   muscle, thymus (young children), skin, bone marrow, peripheral nerve, lung   and melodder cells (CN111363043).

Antibody First Published

Note on publication

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.