Key features and details | |
Cat. No. | MABL-889 |
Name | Anti-CD34 mAbs |
Clone No. | AFD-2E 10 |
From | Recombinant Antibody |
Isotype | Engineer antibody |
Application | WB, FC, IF |
Species Reactivity | Human |
Basic Information | |
Specificity | The antibody is specific to a class III epitope on CD34, which is not susceptible to cleavage by neuraminidase, chymopapain or glycoprotease. |
Alternative Name | Hematopoietic progenitor antigen; Hematopoietic progenitor cell antigen CD34; 2E 10 |
UniProt | P28906 |
Immunogen | The antibody was raised by repeatedly immunising BALB/c mice with KG-1a cells. |
Application Notes | This antibody, labelled with fluoroscein isothiocyanate, has been used in flow cytometry to assess the loss of expression of CD34 epitopes following enzyme incubation (Qian et al., 2008). The antibody has been used as a primary antibody against CD34 in Western blot analysis, using HRP- conjugated goat anti-mouse IgG as a secondary antibody. |
Antibody First Published | Qian et al. Development of new versions of anti-human CD34 monoclonal antibodies with potentially reduced immunogenicity Biochem Biophys Res Commun. 2008 Mar 7;367(2):497-502. PMID：18190781 |
Note on publication | Describes the original generation of this antibody and the construction of chimeric antibodies with human IgG constant regions. |
COA Information （For reference only, actual COA shall prevail） | |
Size | 100 μg Purified antibody. |
Concentration | 1 mg/ml. |
Purification | Protein A affinity purified |
Buffer | PBS with 0.02% Proclin 300. |
Concentration | 1 mg/ml. |
Storage Recommendation | Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C. |
