Anti-CD32 mAbs

Cat. No.

MABL-884

Name

Anti-CD32 mAbs

Clone No.

AFD- MDE-8

From

Recombinant Antibody

Isotype

Engineer antibody

Application

Block, ELISA, FC

Species Reactivity

Human

Basic Information

Specificity

This antibody recognizes and binds Fc-gamma   receptor IIa (FcγRIIa) or CD32a on peripheral blood monocytes,   polymorphonuclear cells and platelets.

Alternative Name

CDw32; Fc-gamma RII-a; FcγRIIa; FcγRII;   FcRII-a; FCGR2A; IgG Fc receptor II-a; Low affinity immunoglobulin gamma Fc   region receptor II-a

UniProt

P12318

Immunogen

The original antibody was generated by immunizing   Human Ig-Tg (Co7) mice with FcgammaRIIa-H131 conjugated to human serum   albumin in complete Freund’s adjuvant.

Application Notes

The binding ability of MDE-8 to FcgammaRIIa   was confirmed using flow cytometry. MDE-8 binds to FcgammaRIIa on cell lines,   peripheral bloodmonocytes, PMN, platelets and blocked ligand binding via this   receptor. It can also effectively blocked FcγRIIa ligand-binding via its   F(ab')2 fragment.Overnight incubation of monocytes with F(ab')2 fragments of   MDE-8 leads to a 60% decrease in cell surface expression of FcγRIIa. MDE-8   whole antibody induced a concomitant 30% decrease of FcγRI on THP-1cells and   monocytes (PMID: 15982355). MDE-8 was reoported to induce Fc-mediated   hypothermia and severe thrombocytopenia in FCGR2A mice (PMID: 26396093).

Antibody First Published

van Royen-Kerkhof et al. A novel human CD32   mAb blocks experimental immune haemolytic anaemia in FcgammaRIIA transgenic   mice. Br J Haematol. (2005); 130(1):130-7.PMID:15982355

Note on publication

Describes the generation and   characterization of the antibody.

COA Information （For reference only, actual COA shall prevail）

Size

100 μg Purified antibody.

Concentration

1 mg/ml.

Purification

Protein A affinity purified

Buffer

PBS with 0.02% Proclin 300.

Concentration

1 mg/ml.

Storage Recommendation

Store at 4⁰C for up to 3 months. For   longer storage, aliquot and store at - 20⁰C.