Cat. No.
MABL-1378
Application
IEM, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
F12
From
Recombinant Antibody
Specificity
F12 recognizes an unknown polypeptide of desmosomal and hemidesmosomal plaques with a MW of 185 kDa (determined from immunoblotting using bovine tongue epithelium extract as the substrate), and by IEM (immunoelectron microscopy) it was determined that it is the intracellular part of the desmosome that is bound (Gilbert, 1997). F12 shares some immunochemical properties with autoantibodies present in paraneoplastic pemphigus sera. Pemphigus diseases are those resulting in blistering of the skin due to the production of autoantibodies against keratinocyte surface proteins - autoantibodies of patients with pemphius vulgaris recognize desmoglein 1 (Dsg1), a major glycoprotein of the desmosome complex.
Alternative Names
UniProt
Immunogen
F12 was established by fusing peripheral blood lymphocytes derived from a human vulgaris patient with a hetermyeloma cell line.
Application Notes
F12 labels the keratinocyte membrane and the basement membrane zone of rat tongue sections by indirect immunofluorescence (IIF). The antibody also stains both the cell membrane and the basement membrane zone of stratified squamous epithelia, and other epithelial tissues such as urinary bladder, small bowel, thymus, and liver. Non-epithelial tissues, such as myocardium are additionally stained. Sera from patients with pemphigus vulgaris can be incubated with human skin sections to determine their capacity to block the binding of F12 in an IIF blocking assay. IEM (immunoelectron microscopy) can be used to determine the ultralocalization of the antibody.
Antibody First Published
Gilbert et al. Production of a Human Monoclonal Anti-epithelial Cell Surface Antibody Derived from a Patient with Pemphigus Vulgaris Journal of Autoimmunity (1992) 5,173-182 PMID:1627232
Note on publication
Describes the generation of an anti-desmosome IgM antibody which recognises a 185 kDa protein which was also recognized by the sera of two patients with pemphigus vulgaris. Its binding properties were studied.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
