Cat. No.
MABL-1332
Application
IP, WB, ELISA, IF
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
1P1
From
Recombinant Antibody
Specificity
This antibody is specific for extracellular domain of the human cytokeratin 18 containing amino acids 'LEDGEDFNLGDALD'. Cytokeratins are intermediate filaments found in the intracytoplasmic cytoskeleton of epithelial tissue. Cytokeratin 18 is widely expressed in human monolayer epithelial tissues, and generally appears in pairs with CK8. CK18 is a serum marker for a variety of tumor diagnosis, as well as a serum marker for liver injury. It is involved in the uptake of thrombin-antithrombin complexes by hepatic cells. When phosphorylated, it plays a role in filament reorganization. It is also involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Alternative Names
CK18; CK-18; CK 18; KRT18; CYK18; Keratin type I cytoskeletal 18; Cytokeratin-18; Cell proliferation-inducing gene 46 protein
UniProt
P05783
Immunogen
The original antibody was generated by immunizing BALB/c mice with human cytokeratin 18 extracellular domain polypeptide 'LEDGEDFNLGDALD' coupled with VLP and the immunogenicity enhancing factor of the traditional KLH.
Application Notes
This antibody can bind human cytokeratin 18 in an ELISA. This antibody was capable of recognizing cytokeratin 18 from PC9 human lung cancer cell lysate in a western blot assay. This antibody was also used in the immunofluorescence staining of PC9 human lung cancer cells fixed with PFA. This antibody can be used in the immunoprecipitation of cytokeratin 18 from the PC9 membrane protein sample.
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
