Cat. No.
MABL-1328
Application
WB, FC, IHC
Isotype
Engineer antibody
Species Reactivity
canine, Rat, Swine, Human
Clone No.
RCK107
From
Recombinant Antibody
Specificity
The antibody reacts exclusively with Cytokeratin 14 which is present in basal cell compartments of stratified and combined epithelia.
Alternative Names
K14; CK-14; Keratin K14; Keratin type I cytoskeletal 14; Cytokeratin-14; Keratin-14
UniProt
P02533
Immunogen
The original antibody was generated by immunizing a mouse with a cytoskeletal preparation of TR146 epithelial cells.
Application Notes
The antibody detected keratin-14 from cytoskeletal extracts were prepared from A431 and HaCaT cell cultures by western blot analysis. This antibody was used for immunohistochemistry studies of keratins on TR146, HaCaT and A431 cells. The antibody stained mostly basal (myoepithelial) cells (Wetzels et al., 1991; PMID： 1705754 and Smedts et al., 1992; PMID： 1379783). Further, the antibody stained mitochondria- rich cells (Spies et al., 1997; PMID： 9440221).
Antibody First Published
Wetzels et al. Basal cell-specific and hyperproliferation-related keratins in human breast cancer Am J Pathol. 1991 Mar;138(3)：751-63. PMID：1705754
Note on publication
The original paper describes the generation and characterization of the antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
