Cat. No.
MABL-1212
Application
food diagnostic, ELISA
Isotype
Engineer antibody
Species Reactivity
Penicillum citreoviridin
Clone No.
5A10
From
Recombinant Antibody
Specificity
This antibody binds citreoviridin, which is one of the mycotoxins that contaminates a wide range of agricultural products, including rice, cereal grains and farm crop products. It is produced by Aspergillus terreus, Eupenicillium ochrosalmoneum and Penicillium citreonigrum. It causes cytotoxicity as an uncompetitive inhibitor of ATP hydrolysis. Consuming food which is contaminated with citreoviridin can cause the disease cardiac beri beri.
Alternative Names
CIT; CTV; Penicillin citreoviridin
UniProt
Immunogen
The original antibody was generated by immunizing BALB/c mice with citreoviridin cross- linked with carrier proteins like BSA and KLH.
Application Notes
The binding reactivity of this antibody for citreoviridin was determined using ELISA. This antibody can be used for detection of citreoviridin in contaminated food samples. Indirect competitive ELISA (ic-ELISA) indicated that the linear range to detect CIT for this antibody was 25-562 ng/mL with IC50 at 120 ng/mL. The limit of detection was 14.7 ng/mL, and the recovery average was (90.612 ± 3.889)% (PMID: 27622814)
Antibody First Published
Wang et al. Screening and Molecular Evolution of a Single Chain Variable Fragment Antibody (scFv) against Citreoviridin Toxin. J Agric Food Chem. 2016 Oct 12;64(40):7640-7648. PMID:27622814
Note on publication
Describes the generation and characterization of this antibody.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
