Cat. No.
MABL-1203
Application
ELISA
Isotype
Engineer antibody
Species Reactivity
Species independent
Clone No.
Chl-D1
From
Recombinant Antibody
Specificity
This antibody binds chloramphenicol and cross reacts with florfenicol, a relate analog to chloramphenicol. Chloramphenicol is a broad spectrum antibiotic used in the treatment of a number of bacterial infections. Chloramphenicol is an antibiotic and is in the class of antimicrobials that inhibits protein synthesis. It has been used for treating superficial eye infections such as bacterial conjunctivitis, and otitis externa. It has also been used for the treatment of typhoid and cholera.
Alternative Names
C11H12Cl2N2O5; Florfenicol; CAS: 56-75-7; CAP; CLM; chloromycetin; chlornitromycin; levomycetin; Chl-DI
UniProt
Immunogen
The original antibody was generated by immunizing a llama with a cocktail of three different small molecules namely chloramphenicol, enrofloxacin and semicarbazide all of which were conjugated with KLH to illicit immune response. A phage display library was generated and the particular clone was isolated by panning the library against chloramphenicol-BSA conjugate.
Application Notes
This antibody can be used for detection of antibiotic adulterants in food. The binding characterization of this antibody for chloramphenicol-BSA was done using ELISA. This antibody was also used in test kit to determine the presence of chloramphenicol in fish extracts. In an indirect competitive ELISA this antibody bound soluble chloramphenicol and florfenicol with an IC50 of 3.202 µM and 3.314 µM respectively. The thermal stability of this antibody determined using a GloMelt™ protein kit and it was reported that the antibody had melting temperatures (TM) over 65°C (PMID: 36153664).
Antibody First Published
Swofford et al. Structure and specificity of an anti‐chloramphenicol single domain antibody for detection of amphenicol residues. Protein Sci. 2022 Nov; 31(11): e4457. PMID:36153664
Note on publication
Describes the generation of single chain antibodies against chloramphenicol, to be used in kits to detect antibiotic adulteration in seafoods.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
