Cat. No.
MABL-1173
Application
WB, ELISA, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
12B6
From
Recombinant Antibody
Specificity
The antibody recognises human CDX2. The CDX2 protein is a homeobox transcription factor expressed in the nuclei of intestinal epithelial cells, playing an essential role in the development and function of the digestive system.
Alternative Names
Homeobox protein CDX-2; CDX-3; Caudal-type homeobox protein 2; rabbit mono-ERP2764Y; TA347
UniProt
Q99626
Immunogen
The original antibody was generated by immunizing a mouse with the CDX2-KLH protein.
Application Notes
The antibody was used for immunohistochemistry on stomach adenocarcinoma, showing accurate staining location, clear staining, no non-specific staining, and clean background. Further, immunohistochemistry staining on stomach adenocarcinoma from 12B6 antibody and the commercial antibody, rabbit mono-ERP2764Y, were compared. Results showed the staining intensity of 12B6 antibody was significantly higher than those of ERP2764Y, showing 12B6 antibody is more sensitive. Moreover, the antibody and the commercial antibody TA347 were used for immunohistochemistry on appendix tissue. Results showed that the antibody (12B6) and the commercially available antibody (TA347) were simultaneously detected on the normal tissue chip, and the negative and positive detection results were consistent, indicating that the specificity of the antibody in normal tissue was equivalent to that of the commercially available antibody. The antibody detected human CDX2 by western blot analysis (CN113265000).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
