Cat. No.
MABL-1160
Application
activation, functional assays, in vitro, ELISA, FC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
BA143
From
Recombinant Antibody
Specificity
This antibody is specific for Human CD96 and does not cross-react with Monkey CD96.
Alternative Names
Tactile; T cell activation, increased late expression; Cluster of Differentiation 96
UniProt
P40200
Immunogen
The original antibody was generated by immunizing mice with human CD96.
Application Notes
The original antibody (Mouse IgG1) demonstrated high-affinity binding to CHO cells expressing human CD96 isoform 2 (EC50 = 5.9 ng/mL), as validated in an ELISA. It effectively blocked CD155-Fc binding to the same target (IC50 = 152.4 ng/mL) in a competitive binding assay. It also exhibited binding to CHO cells expressing the V/V variant of human FcγRIIIa (EC50 = 191.5 ng/mL), confirmed through an ELISA. In an in vitro T cell activation assay, the antibody successfully bound to activated CD4+ and CD8+ T cells from three donors, and in a staphylococcal enterotoxin A (SEA)-stimulation assay, it showed limited functional activity in promoting IL-2 secretion by PBMCs. Additionally, it displayed a binding affinity (KD = 13.3 nM) for the full-length extracellular domain of human CD96 in a kinetic analysis (US20240141039A1).
Antibody First Published
Note on publication
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
