Cat. No.
MABL-1158
Application
WB, IF, IHC
Isotype
Engineer antibody
Species Reactivity
Human
Clone No.
LOB3-11
From
Recombinant Antibody
Specificity
This antibody is specific for human CD95. CD95 is a protein that has an important role in the apoptosis of cells. Due to this property is can play a role in cancer.
Alternative Names
Tumor necrosis factor receptor superfamily member 6; FAS; Apo-1 antigen; Apoptosis-mediating surface antigen FAS; FASLG receptor
UniProt
Immunogen
The mouse version of this antibody was raised by immunizing a mouse with a fusion protein comprising extracellular domain of human Fas linked to human Fc.
Application Notes
To determine the involvement of caspases in apoptosis induced by CD20, a western blot was performed using the mouse version on this antibody on Daudi and Raji cells treated with anti-CD20. Furthermore, the effect of this antibody on mitochondrial permeability was determined. An increase in permeability was indeed observed after treatment with this antibody (Chan et al, 2003; pmid：14500384).Cytospin preparations from MOLT-4 and Jurkat T-cells were stained for immunofluorescence using the mouse version of this antibody to study whether gal-1 interferes with the Fas-associated apoptosis cascade in the T-cell line (Brandt et al, 2008; pmid：18288482). To study whether the expression of the proapoptotic Fas/FasL in the implantation site in impaired during abortion, placental tissue from woman with either voluntary or involuntary abortion was stained for immunohistochemistry using the mouse version of this antibody (Minas et al, 2007; pmid;17704118).
Antibody First Published
Chan et al. CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts Cancer Res. 2003 Sep 1;63(17):5480-9. PMID:14500384
Note on publication
D20-induced apoptosis was investigated with a panel of anti-CD20 monoclonal antibodies (mAb) in a wide range of cell lines.
Size
100 μg Purified antibody.
Concentration
1 mg/ml.
Purification
Protein A affinity purified
Buffer
PBS with 0.02% Proclin 300.
Storage Recommendation
Store at 4⁰C for up to 3 months. For longer storage, aliquot and store at - 20⁰C.
